The Basic Principles Of high performance liquid chromatography
The Basic Principles Of high performance liquid chromatography
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The detector screens the mobile section exiting the column and generates a sign according to the presence and quantity of analytes eluting. Widespread detector sorts consist of:
Ion-Trade: Separates charged molecules primarily based on their own conversation with charged functional teams around the stationary stage.
전자를 '고정상', 후자를 '이동상'이라 부르며 크로마토그래피에서는 분석자는 고정상과 이동상의 조합에 의해 분석물의 분리를 제어할 수 있게 됩니다.따라서 분석물, 고정상, 이동상, 세 가지 특성의 이해가 크로마트그래피에서 매우 중요합니다.
Lowering the level of acetonitrile and raising the amount of drinking water from the mobile will maximize retention times, supplying a lot more time for you to effect a separation.
The selection on the column form will depend on the physicochemical properties of your analytes currently being divided.
we realized how to adjust the cellular section’s polarity by Mixing jointly two solvents. A polarity index, even so, is simply a information, and binary cellular stage mixtures with similar polarity indices may well not resolve equally a set of solutes. Table 12.5.2
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Acid–base chemistry isn't the only example of a secondary equilibrium reaction. Other illustrations include ion-pairing, complexation, and the interaction of solutes with micelles. We're going to evaluate the last of these in Chapter 12.seven after we discuss micellar electrokinetic capillary chromatography.
Polarity: The polarity of your cellular period substantially influences separation. A more polar mobile phase interacts extra strongly with polar analytes, creating them to elute (exit the column) slower than much less polar analytes.
원하는 분석 결과를 얻기 위해서는 컬럼도 충분히 고려하고 선택하는 것이 좋습니다.
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Frequent mobile period modifiers like acids and bases might be additional to great-tune the conversation in between analytes plus the column. These modifiers can:
The choice of detector depends upon the particular demands of your analysis, considering elements like sensitivity, selectivity, and compatibility Together with the cellular stage.
, which is the greater prevalent type of HPLC, the stationary section is nonpolar along with the mobile phase is polar. The most common nonpolar stationary phases use an organochlorosilane where the R group can be an n